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  • . . . about BioMed Central, help us by answering 3 questions Research article Open Access Heterogeneous pattern of DNA methylation in developmentally important genes correlates with its chromatin conformation Puja Sinha 1 , Kiran Singh 2 and Manisha Sachan 1 Email author BMC Molecular Biology BMC series – open, inclusive and trusted 2017 18 :1 . . .
  • . . . Author(s) 2017 Received: 8 October 2016 Accepted: 13 December 2016 Published: 11 January 2017 Abstract Background DNA methylation is a major epigenetic modification, playing a crucial role in the development and differentiation of higher organisms. DNA methylation is also known to regulate transcription by gene repression
  • . . . Molecular Biology 2017 18 :2 Published on: 31 January 2017 Full Text PDF Research article Heterogeneous pattern of DNA methylation in developmentally important genes correlates with its chromatin conformation DNA methylation is a major epigenetic modification, playing a crucial role in the development and differentiation of higher organisms.
  • . . . on: 11 January 2017 Full Text PDF Research article Novel insight into the composition of human single-stranded DNA -binding protein 1 (hSSB1)-containing protein complexes Single-stranded DNA -binding proteins are essential cellular components required for the protection, metabolism and processing of single-stranded DNA
  • . . . Abstract Background Cells respond to numerous internal and external stresses, such as heat, cold, oxidative stress, DNA damage, and osmotic pressure changes.
  • . . . preference mRNA induction Background UV damage causes lesions in the genome that interfere with cellular DNA metabolism and lead to cell death
  • . Abrogation of two from among of the three C/EBP DNA-binding motifs of the promoter revealed that C/EBPα acts in cis but C/EBPβ in trans .
  • . . . through epigenetic mechanisms, since the SCD1 activity was found to correlate with the degree of CpG island DNA-methylation at the promoters of relevant immune genes [ 23 ]. Hormonal and nutrient factors are known to regulate SCD1 expression in several species including man and mouse [ 24 , 25 ]
  • e principle of 3C is to convert physical chromatin interactions into specific DNA ligation products, which are then detected by quantitative polymerase chain reaction (qPCR).
  • During dPCR, DNA samples are partitioned into thousands or millions of individual PCR reactions
  • . . . Sox transcription factors is defined by the presence of a conserved high mobility-group (HMG) domain that mediates DNA-binding and is highly similar to that of the sex-determining region (SRY) protein [ 1 , 2 ].
  • SOX proteins exert gene activation or repression by binding to a consensus DNA motif, with or without aid of other transcription factors [ 1 , 4 ]
  • . . . (DEspR, formerly Dear ) as a unitary transcribed pseudogene due to a stop [TGA]-codon at codon#14 in automated DNA and RNA sequences.
  • . . . criteria for demonstration of a protein’s existence at the highest (protein) level, which a priori, would override DNA- or RNA-based deductions. Methods To dissect the nucleotide sequence discrepancy, we performed Maxam–Gilbert sequencing and reviewed 727 RNA-seq entries
  • e reliability of the method was tested using mixtures of cDNA templates as well as RNA samples from different sources.
  • Finally, the method was based on time consuming cDNA standard curves and different amplification efficiencies of the reactions were not considered. Here we present an improved and reliable method to quantify the ratio of splice variants in a single sample based on RT-qPCR
  • . . . Dicer is not only involved in miRNA biogenesis, but also plays a role in other cellular processes such as genomic DNA fragmentation during apoptosis, the processing of endogenous siRNA and the detoxification of repeat elements [ 5 – 8 ].
  • . . . knockout mice, after RNA extraction from the whole kidney the RT reaction was performed using the high-capacity cDNA Kit (Life Technologies, Carlsbad, California, USA)
  • . . . Molecular Biology is an open access journal publishing original peer-reviewed research articles in all aspects of DNA and RNA in a cellular context, encompassing investigations of chromatin, replication, recombination, mutation, repair, transcription, translation and RNA processing and function.
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  • e FPS cDNA sequences from 68 species were used to reconstruct a phylogenetic tree.
  • . . . Gynostemma pentaphyllum (GenBank accession KJ917160) were cloned by our laboratory using rapid-amplification of cDNA ends (RACE) technology, and other cDNA sequences for FPS genes were collected from existing databases
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