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  • Cas9 endonuclease complexed with a crRNA and separate tracrRNA cleaves foreign DNA containing a 20-nucleotide crRNA complementary sequence adjacent to the PAM sequence.
  • Genome engineering with Cas9 Nuclease Wild-type Cas9 nuclease site specifically cleaves double-stranded DNA activating double-strand break repair machinery. In the absence of a homologous repair template non-homologous end joining can result in indels disrupting the target sequence
  • Traditionally, changes in DNA which are associated with antibiotic resistance in S.
  • This approach was recently used to identify the single nucleotide polymorphisms (SNPs) in DNA that may confer beta-lactam resistance in S. pneumoniae 6 . However, understanding how genetic variations contribute to antibiotics resistance remains underexplored
  • . . . The ‘eureka’ moment that revolutionised crime solving Story In 1984, Alec Jeffreys developed the technique of DNA fingerprinting in his laboratory at the University of Leicester.
  • The discovery of DNA Story How was DNA first discovered and who discovered it? Read on to find out How is pharmacogenomics being used? Story In a small number of cases, doctors are able to use pharmacogenomics in their treatment of . . .
  • . . . DNA Structure Activity Problem 7: Are the two strands of DNA parallel or anti-parallel? Are the two strands of DNA parallel or anti-parallel? Switch to the view labeled "Antiparallel DNA strands".
  • . . . Bar can be used for zooming in and out. Using the red oxygen atoms as a reference, determine if the two strands of DNA spiral in the: A. same direction. The strands are "parallel". B. opposite direction. The strands are "antiparallel"
  • . . . DNA Structure Activity Problem 1: Identifying the location of phosphate groups.
  • Next, identify the location of the phosphate groups in the overall view of DNA labeled "original". Use the " " zoom tool to zoom in and get a close look, and then click on "original" to return to the starting view. Where are phosphates located in DNA? A. the outermost surface of the DNA B
  • . . . DNA Structure Activity Problem 3: Grooves in the DNA double helix Examine the view labeled "phosphate group location".
  • . . . Proteins that interact with DNA often make contact in these grooves. How would you describe the grooves in the DNA surface? A. a single groove of uniform size B. a single groove that varies in size C. two distinct grooves, both of the same size D
  • . . . DNA Structure Activity Problem 7: Are the two strands of DNA parallel or anti-parallel? Correct! Are the two strands of DNA parallel or anti-parallel? Switch to the view labeled "Antiparallel DNA strands".
  • . . . Bar can be used for zooming in and out. Using the red oxygen atoms as a reference, determine if the two strands of DNA spiral in the: A. same direction. The strands are "parallel". B. opposite direction. The strands are "antiparallel"
  • . . . DNA Structure Activity Problem 1: Identifying the location of phosphate groups.
  • Phosphates alternate with deoxyribose sugars to form the DNA backbone. This view emphasizes the location of the phosphates on the outside of the DNA helix
  • . . . DNA Structure Activity Problem 6: Location of the sugar in the DNA double helix Switch to the view of DNA labeled "Sugar location." In this view, the carbon atoms of the sugars are colored green to emphasize their location.
  • . . . The " " zoom tool from the Toolbar can be used for a closer look at the position of individual sugar rings in the DNA structure. Are the deoxyribose sugars, and the phosphates that link them together, located: A. stacked atop each other in the middle of the DNA helix. B. on the outside surface, interspersed with phosphate groups. C
  • . . . DNA Structure Activity Problem 3: Grooves in the DNA double helix Examine the view labeled"phosphate group location".
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  • . . . DNA Structure Activity Problem 3: Grooves in the DNA double helix Correct! Examine the view labeled"phosphate group location".
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