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  • . . . restriction enzymes? Research Spotlight Behind the paper: NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases.
  • More Restriction enzymes cut DNA at specific sequences, and their discovery helped usher in the era of molecular cloning. These days, they are being used for much more than traditional cloning. Learn more in this episode of NEB TV
URL: https://www.neb.com - 49.5 kb
  • . . . THEORY OF AGING MITOCHONDRIA AND AGING THE GLYCATION THEORY OF AGING PROTEINS DAMAGE AND MAINTENANCE IN AGING DNA DAMAGE AND DNA REPAIR TELOMERES AND AGING CELLULAR SENESCENCE AND APOPTOSIS IN AGING "ACCELERATED AGING" DISEASES (SEGMENTAL PROGERIA) LONGEVITY GENES (FLIES WORMS) LONGEVITY GENES (MAMMALS) SIRTUINS AND DEACETYLASES IN . . .
  • Aging damage occurs to molecules (DNA , proteins, lipids), to cells and to organs
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  • . . . a multivalent cellular signal that regulates multitude of physiological and pathophysiological processes including DNA repair, inflammation, cancer, infection and proteasomal degradation.
  • . . . the structure and mechanism of the enzymes and molecular switches that carry out cellular signal transduction and DNA replication
  • . . . the cell Methods Technology Search form Search In: Debates Society and Behaviour Is it ethical to have a national DNA database? The National DNA Database has proved to be a valuable tool in the fight against crime.
  • The UK National DNA Database holds the DNA profiles and relevant DNA samples from a select number of UK individuals. It is the largest database of its kind in the world and is continuing to grow each year
  • . . . Gene ontology Molecular function • protein N-terminus binding • transcription factor activity, sequence-specific DNA binding • protein self-association • core promoter sequence-specific DNA binding • transcription regulatory region DNA binding • RNA polymerase II transcription factor activity, sequence-specific DNA binding • protein . . .
  • . . . RNA polymerase II transcription factor binding • zinc ion binding • chromatin binding • protease binding • damaged DNA binding • protein binding • histone acetyltransferase binding • copper ion binding • protein kinase binding • DNA binding • chaperone binding • transcriptional activator activity, RNA polymerase II transcription . . .
  • . . . in the synthesis of a functional gene product such as a protein • transcription – the process of making RNA from a DNA template by RNA polymerase • transcription factor – a protein that binds to DNA and regulates gene expression by promoting or suppressing transcription • transcriptional regulation – controlling the rate of gene transcription . . .
  • . . . – the process of making RNA from a DNA template by RNA polymerase • transcription factor – a protein that binds to DNA and regulates gene expression by promoting or suppressing transcription • transcriptional regulation – controlling the rate of gene transcription for example by helping or hindering RNA polymerase binding to DNA • upregulation . . .
  • . . . External IDs OMIM: 194360 MGI: 99137 HomoloGene: 31368 GeneCards: XRCC1 Gene ontology Molecular function • damaged DNA binding • enzyme binding • protein binding • DNA ligase activity • 3' overhang single-stranded DNA endodeoxyribonuclease activity Cellular component • nucleoplasm • nuclear chromosome, telomeric region • ERCC4-ERCC1 complex • . . .
  • . . . • ERCC4-ERCC1 complex • nucleus Biological process • single strand break repair • nucleotide-excision repair, DNA gap filling • transcription-coupled nucleotide-excision repair • response to hypoxia • response to organic substance • double-strand break repair via homologous recombination • response to drug • cellular response to DNA . . .
  • . . . Skip to navigation Return to homepage Welcome, Guest Sign In or Sign Up My Account My NEB Quick Order 0 items DNA Amplification, PCR qPCR Product Listing Application Overview In order to study or detect individual genes or specific DNA regions or mutations of interest, it is often necessary to obtain a large quantity of nucleic acid . . .
  • Rather than isolate a single copy of the target DNA from a large number of cells, it is often more useful to generate multiple copies of a target from a single molecule of DNA or mRNA, via an in vitro amplification method
  • . . . about BioMed Central, help us by answering 3 questions Research article Open Access Heterogeneous pattern of DNA methylation in developmentally important genes correlates with its chromatin conformation Puja Sinha 1 , Kiran Singh 2 and Manisha Sachan 1 Email author BMC Molecular Biology BMC series – open, inclusive and trusted 2017 18 :1 . . .
  • . . . Author(s) 2017 Received: 8 October 2016 Accepted: 13 December 2016 Published: 11 January 2017 Abstract Background DNA methylation is a major epigenetic modification, playing a crucial role in the development and differentiation of higher organisms. DNA methylation is also known to regulate transcription by gene repression
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  • . . . Skip to navigation Return to homepage Welcome, Guest Sign In or Sign Up My Account My NEB Quick Order 0 items DNA Modification Product Listing Application Overview The preparation of DNA for molecular applications often requires sample processing beyond restriction enzyme digestion, fragmentation or PCR amplification.
  • Modifying enzymes can be used to manipulate or repair DNA for downstream applications, such as: Recombinant DNA assembly for subsequent cloning PCR amplification DNA labeling or probing Sequencing While there are many modifying enzymes that act on DNA to prepare it for a variety . . .
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